To accommodate the increase in demand for single cell technologies, we are seeking a self-motivated, reliable and experienced research assistant to join the Institute's Next Generation Sequencing (NGS) facility. This core facility supports the NGS needs of our researchers, and is currently expanding into delivering services of other single cell requirements. We are seeking a molecular biologist with proven skills in RNA/DNA manipulation and a working knowledge of NGS sequencing technologies to support the 10X chromium droplet-based technology in the facility. The applicant will assist and work closely together with the facility manager. Routine tasks will include: receiving samples from users, performing single cell library preparations on the 10X chromium platform, facilitating the access and usage of specialised single cell-based equipment, QC of RNA and DNA samples, library construction for standard NGS library protocols, handling processes on Covaris, Bioanalyzer and qPCR machines, troubleshooting and correction of suboptimal results. Requirements: A Bachelor of Science degree in a relevant discipline, such as molecular biology, genetics, biochemistry, or similar.Superior laboratory skills in Molecular Biology, Genetics, Biochemistry, or related areas, preferably with some experience in NGS technology and its application.Solid knowledge and hands-on experience with molecular biological methods such as DNA and RNA isolation, cDNA synthesis, enzymatic manipulation of DNA and RNA, PCR.A working knowledge of NGS technology is essential.Ability to quickly learn and independently apply new techniques after initial training.Excellent organizational, documentation and time management skills to ensure successful teamwork, and a high level of attention to detail.Previous experience in a service role would be an advantage.Prior experience of cell culture would also be advantageous, but experience in stem cell biology is not necessary. The successful applicant will receive all necessary training, and work in close collaboration with the Facility Manager, the Bioinformatics team, and researchers engaged in various NGS projects. Fixed-term: The funds for this post are available until 30 June 2022 in the first instance. Once an offer of employment has been accepted, the successful candidate will be required to undergo a health assessment. Click the 'Apply' button below to register an account with our recruitment system (if you have not already) and apply online. CClosing day for all applications is Sunday 21 February 2021, with interview provisionally scheduled for Thursday 04 March 2021. Please quote reference PS25514 on your application and in any correspondence about this vacancy. The University actively supports equality, diversity and inclusion and encourages applications from all sections of society. The University has a responsibility to ensure that all employees are eligible to live and work in the UK.
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